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Go with the Flow

The BD FACSAria™ is a state-of-the-art flow cytometer that integrates advanced digital electronics with a fixed-alignment optical path, allowing real-time data acquisition and analysis.  The FACSAria is equipped with 3 solid state lasers, 9 fluorescent PMT detectors, 2 scatter detectors, an aerosol management system and multiple sort acquisition modes.

Optical Light Configuration

Optical Light Configuration Chart

By knowing the optical configuration of the instrument you can choose what fluorochromes or dyes will be detected.  Besides the common ones listed, you can use one of the on-line spectral viewers or product inserts to find the excitation/emission wavelengths of the fluorochome in question.  Then you compare these curves to the above configuration to see how likely detection will be and how much spectral overlap to expect.

To view the online spectral viewer please click on the following links:

BD Spectral Viewer

Invitrogen Spectral Viewer

BioLegend Specral Viewer

eBioscience Spectral Viewer

Services available on the FACSAria:

  • Single or multi-color cellular analysis (phenotyping, intracellular staining, etc.)
  • Sorting – up to four different subsets at a time
  • Sorting into plates – single cell up to any stated number of cells sorted into a variety of plates (6-well, 96-well, etc.)
  • DNA/Cell cycle analysis
  • Calcium Flux studies

Scheduling and Sample Preparation:

  • Signups are on a first-come, first-served basis, scheduled on an on-line calendar.  Please consult with Core Staff before your first visit.
  • All samples run on the Aria must be pre-filtered to avoid clogging the nozzle.  We recommend using 35um filter top tubes (Falcon 352235, VWR order# 21008-948.) 
  • Prepare appropriate controls including negatives, single stained controls for compensation, isotypes and/or FMO’s (Fluorescence Minus One), as well as biological controls.  If in doubt, please consult with Core staff on experiment design.
  • Sorts – Please download and read our Sorting Guidelines (PDF) or pick up a copy from the Core.

Publication Acknowledgement Request (Click Here)

  • We would appreciate a reprint of your publications.