Sub groups of graphene nanoparticles have been increasing in popularity for commercial applications due to the particle’s unique chemical properties and low-cost. This study evaluated the biotoxicity of reduced graphene oxide (rGO) and graphene oxide (GO) particles on C10 lung epithelial cells and C57BL/6 alveolar macrophages isolated from mice. Evaluations included measurements of cell viability and cell death, along with the release of proinflammatory cytokines from both cells.