Nanoparticles are increasingly utilized in multiple fields such as but not limited to, medicine, clothing, agriculture, and industry. Carbon nanotubes have important uses in many of these fields so understanding their potential for adverse effects is vital. Macrophages are a key component of the immune system and are known to separate into functional subsets that generate distinct responses in the body. Pulmonary macrophages, key regulators of immune responses to respiratory exposures, have not yet fully been separated in subsets.